FIG. 7.

Nox4 involvement in UVB-induced ROS generation. (A) UVB preconditioning did not induce the ROS generation in Nox4-silenced ASCs, as shown by DCF-DA fluorescence microscopy. (B, C) Nox4 mRNA and protein expression levels were both significantly increased by UVB treatment, demonstrated by RT-PCR (B) and western blot analysis (C), respectively. (D, E) Inhibition of UVB-stimulated (20 mJ/cm²) ROS generation by DPI treatment (100 μM). DPI significantly downregulated the mRNA (D) and protein (E) expression of Nox4.