Table 4.
Expected Fluorescence Phenotype Progeny for a “YES” Cross
| Parental Gentotypes: GloGFP/Glo- (Lime Green) X GloBFP/Glo- (Blue) | |||
|---|---|---|---|
| |
Excitation wavelength* |
||
| Progeny genotype | 350 nm | 480 nm | 545 nm |
| GloGFP/Glo- | Will not fluoresce | Will fluoresce | Will not fluoresce** |
| GloBFP/Glo- | Will fluoresce | Will not fluoresce | Will not fluoresce |
| GloGFP /Glo-; GloBFP/Glo- | Will fluoresce | Will fluoresce | Will not fluoresce** |
| Neither GloGFP nor GloBFP | Will not fluoresce | Will not fluoresce | Will not fluoresce |
A band of wavelengths was used, and the peak wavelength is listed; **will have only very dim fluorescence.
Note that each genotype gives a different fluorescence pattern. See Table 3 for further explanation of a “YES” cross.