Table 5.
Expected Fluorescence Phenotype Progeny for a “NO” Cross
| Parental genotypes: GloYFP/Glo- (Yellow) X GloRFP/Glo- (Red) | |||
|---|---|---|---|
| |
Excitation wavelength* |
||
| Progeny genotypes | 350 nm | 480 nm | 545 nm |
| GloYFP/Glo- | Will not fluoresce | Will fluoresce | Will fluoresce |
| GloRFP/Glo- | Will not fluoresce | Will not fluoresce | Will fluoresce |
| GloYFP/Glo-; GloRFP/Glo- | Will not fluoresce | Will fluoresce | Will fluoresce |
| Neither GloYFP nor GloRFP | Will not fluoresce | Will not fluoresce | Will not fluoresce |
A band of wavelengths was used, and the peak wavelength is listed.
Note that every different genotype does not have a distinct fluorescence pattern. See Table 3 for further explanation of a “NO” cross.