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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: Methods Mol Biol. 2011;735:47–54. doi: 10.1007/978-1-61779-092-8_5
8× buffer M 3.125 µl
Annealed template oligo 1 µl  
1.25 mM dAdT (final 50 µM) 1 µl
dH2O 13.875 µl
[α-32P]dCTP 5 µl
Klenow large fragment 1 µl
      Total 25 µl