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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Eur J Immunol. 2012 Mar;42(3):651–661. doi: 10.1002/eji.201141581

Figure 1. Generation of Tim-1 deficient mice.

Figure 1

A. Schematic of knockout allele. B. Genotyping PCR of Tim-1 wild-type, heterozygous, and knockout alleles. C. FACS staining of splenocytes from BALB/c wild-type or Tim-1 deficient mice activated for 72 hours with 10 μg/mL anti-IgM. CD19+ gated cells were stained with anti-Tim1 (black line) or isotype control (shaded histogram). D. Quantitative PCR analysis of Tim-1, Tim-2, Tim-3 and Itk in Tim-1 KO and WT mouse CD4+ T cells. Results are representative of three (Tim-1, Tim-2, Tim-3) or two (Itk) independent experiments. E. Surface expression of Tim-4 on WT and Tim-1 KO peritoneal cells via FACs analysis. Results are representative of two independent experiments. Statistical analysis was prepared with unpaired two-tailed t tests. Error bars represent standard error of mean. * p<0.05, ** p<0.01, *** p<0.001.