Figure 7. CR3 KO mice have sustained deficits in eye-specific segregation.

A, Representative image of a P30 WT (left) demonstrates minimal overlap (yellow) between ipsilateral (red) and contralateral (green) RGC inputs. Indicative of a synaptic pruning deficit, CR3 KO mice (right) had increased overlap (yellow) of ipsilateral (red) and contralateral (green) RGC inputs. Scale bar = 100 µm. B–C, P10 (B) and P30 (C) CR3 KO mice had statistically significant, threshold-independent deficits in retinogeniculate pruning. D, The percentage of ipsilateral territory is significantly increased in P30 CR3 KO mice as compared to WT littermate controls. E, Although trending towards an increase, there is no statistically significant difference in percentage of contralateral territory. F, G dLGN from P30 CR3 WT (F) or KO (G) mice, dotted line boxes in lower magnification image (left panels) correspond to ipsilateral region magnified in middle panels (yellow i–ii) or contralateral region magnified in far right panels (white i–ii). Bottom panels in F,G (ii) are contralateral (CTB-488, green, left panel) channel or ipislateral (CTB-594, red, right panel) alone. G, There were increased aberrant contralateral projections (middle panel; i, green and ii) within the ipsilateral territory in P30 CR3 KO mice as compared to WT littermates (F, middle panel). Similarly, there were aberrant ipsilateral projections (right panel; i, red and ii) within contralateral regions of the dLGN in CR3 KO mice as compared to WT littermates (F, right panel). Left panels, scale bar = 100 µm. Middle and right panels, scale bar = 10 µm. B–C, *P<0.0001 by Student’s t-test, n=6 (P10) or 4 (P30) mice/genotype. D, *P<0.03 by Student’s t-test, n=4 mice/genotype. All error bars represent s.e.m. See also Figure S6.