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. 2012 Dec 5;4(12):1451–1467. doi: 10.3390/toxins4121451

Figure 1.

Figure 1

Magnitude of LF titer is more dependent on MHC II than that of PA titer after immunization. End-point serum IgG antibody titers to PA (a) or LF (b) in sera of groups of mice who had received three immunizations of rPA (a) or rLF (b) as determined by ELISA are shown. Positivity was defined as OD ≥ 3 SD above the average OD of pre-immunized serum diluted 1:100 from the same animals. Average OD (± SD) for pre-immunized mouse sera tested against PA was 0.068 ± 0.007 for A/J mice, 0.090 ± 0.023 for B6.H2k mice, and 0.120 ± 0.021 for B6 mice. Average OD (± SD) for pre-immunized mouse sera tested against LF was 0.102 ± 0.01 for A/J mice, 0.118 ± 0.01 for B6.H2k mice, and 0.119 ± 0.01 for B6 mice. Shown is the last titer at which each animal’s serum was positive, with the median for each group. *** p < 0.001; * p < 0.05 (Kruskal-Wallis test with Dunn’s post-hoc comparison). Data show combined results from two separate experiments.