Figure 7.

CaMKII inhbitor KN93 regulates post-translational modification of SOD1. Hepatocytes were pre-incubated without (A, B) or with 30 µM KN93 (C, D) prior to addition of nodularin (200 nM). After 10 min of exposure nodularin samples (B, C) and controls (A, D) were TCA-precipitated and proteins were separated by two-dimensional gel electrophoresis. Gels were blotted and the membranes were stained with Ponceau S (E–H) before they were probed with anti-SOD1 antibody. SOD1 spots were horizontally aligned based on the Ponceau S staining. Positions of N1, P1 and P2 (Figure 2) are indicated.