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SOD1 is highly concentrated below the cellular membrane in hepatocytes and also in the apoptotic cell membrane buds. In untreated control cells, high concentration of SOD1 was found in the sub-membranous area (A). After 2 min of nodularin treatment (5 µM) SOD1 localized to the apoptotic cellular membrane buds and stretched into the cytosol (B and insert of B). To visualize SOD1 cells were immunostained with anti-SOD1 antibody. Bars: 10 µm.
