Table 1.
Fluorescent-activated cell sorting (FACS) analysis of peripheral blood mononuclear cell (PBMC) lymphoid antigen expression.
| (a) Markers of PHA/IL-2 activated PBMC lymphoid subsets | |||||
| Antigen | % positive | ||||
| CD3-T cells | 97.4 | ||||
| CD14-monocytes | 2.0 | ||||
| CD19-B cells | 1.5 | ||||
| (b) Cell surface marker labeling of VT treated PHA/IL-2 activated PBMCs | |||||
| Treatment | %CD3+ve | ||||
| Untreated | 97.4 | ||||
| 1 µg/mL VT1 | 95.7 | ||||
| 1 µg/mL VT1A | 94.2 | ||||
| 1 µg/mL VT1B | 97.9 | ||||
| (c) Effect of VT1A treatment on CD4 positive vs. CD8 positive T cell composition | |||||
| Treatment | % CD3+ T cells * | % CD4+ CD3+ T cells * | % CD8+ CD3+ T cells * | CD4+ CD8+ T cells * | |
| Untreated Control | 85.1 | 41.7 | 47.0 | 0.9 | |
| 1 µg/mL VT1A | 88.1 | 43.5 | 49.2 | 0.9 | |
* Different T cell antigen markers.