Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Dec 19;4(12):1582–1600. doi: 10.3390/toxins4121582

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Thin layer chromatography of acetone extracts of two separate A. parasiticus SRRC2043 ΔaflJ transformants (the wild-type accumulates O-methylsterigmatocystin) grown on YES medium for 48 h. Complementation of aflJ function was tested on A. parasiticus ΔaflJ transformed with plasmid pPTRI-gpdA-aflJx-trpC where aflJx equals one of the tested aflJ gene homologs from A. flavus aflatoxin cluster (fla), A. nidulans sterigmatocystin cluster (nid AN7223), A. nidulans monodictyphenone cluster (nid AN11201), D. septosporum dothistromin biosynthesis cluster aflJ (doth, R. Bradshaw, personal comunication). The ΔaflJ mutant (aflJko) was included as the negative control. Two separate transformants were tested for each study. OMST is the O-methylsterigmatocystin standard (Sigma). Elution buffer was toluene:ethyl acetate:acetic acid (6.5:3.5:1). Cultures were extracted after 48 h growth on PDA plates.