Skip to main content
. 2012 Dec 19;4(12):1582–1600. doi: 10.3390/toxins4121582

Figure 3.

Figure 3

End-point RT-PCR studies. (A) RT-PCR was done on total RNA from fungal cultures grown on PDA medium for 48 h. The fungal cultures were A. parasiticus SRRC2043 ΔaflJ before and after transformation with the plasmid, pPTRI-gpdA-aflJx-trpC plasmid where aflJx stands for Dothistroma septosporum aflJ (Ds), A. nidulans AN7223 aflJ (Anid), A. flavus aflJ AAS90096 (Af). RT-PCR involved reverse transcription followed by PCR (oligonucleotide primers listed in Supplementary Materials) whereas the No RT controls used the same primer sets but omitted the reverse transcription step. The aflatoxin biosynthesis genes tested for expression were hexA, pksA, omtA, and ver-1; (B) RT-PCR was done using primers to Dothistroma septosporum aflJ introduced by transformation into A. parasiticus SRRC2043. In all cases the oligonucleotide primers sequences flanked an intron. The smaller PCR product, marked by arrows reflects amplification of the cDNA whereas the larger PCR product could be PCR from either genomic DNA or unprocessed transcript.