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. 2012 Dec 19;4(12):1582–1600. doi: 10.3390/toxins4121582

Figure 4.

Figure 4

Chromatin immunoprecipitation (ChIP) to test AflJ and AflR binding to known AflR-binding sites [14]. Transformants of A. flavus CA14 harbored genes expressing N-terminal c-Myc-tagged AflR or AflJ. (A) Detection by PCR with oligonucleotides to either hexB, ver-1, aflJ, or pksA of chromatin DNA immunoprecipitated by anti-c-Myc in transformants expressing c-Myc-tagged AflJ; (B) Similar detection by PCR with the same oligonucleotides of chromatin DNA immunoprecipitated by anti-c-Myc in transformants expressing c-Myc-tagged AflR. Negative controls for both studies were DNAs isolated by ChIP in the absence of c-Myc antibody. The Y-axis (relative enrichment) is the ratio of the amount of bound DNA to input DNA given in arbitrary units.