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. 2012 Dec 19;4(12):1582–1600. doi: 10.3390/toxins4121582

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Comparison of vesicle/endosome distribution in the A. parasiticus ΔaflJ mutant grown in the dark for 44 h and 68 h on YES liquid medium. (A) Morphology of ΔaflJ was evaluated at indicated time points using bright field microscopy; (B) Binary images of the corresponding distribution of vesicles and endosomes in wild-type A. parasiticus and ΔaflJ; (C) Descriptive statistics of size and distribution of the vesicles/endosomes in Figure 5B measured by CMEIAS digital image analysis [32]. The measurement features are defined in Supplementary information.