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Binding of native hMPV F protein to heparin. Start material (ST), flow through (FT), wash (W) and 2M NaCl elution (E) fractions were analysed by 10% SDS-PAGE under reducing conditions and western blot analysis using anti-hMPV F antibody. Arrows indicate bands corresponding to the predicted sizes of full length precursor hMPV-F (F0) and the cleavage fragment hMPV F1. Molecular weight markers are shown in kDa.
