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. 2013 Jan;27(1):51–62. doi: 10.1096/fj.12-211920

Figure 5.

Figure 5.

Effect of neurotrophic signals on the developmental shortening of mitochondria. A–D) After electroporation of DsRed-mito in E3 chick spinal cord, vehicle control (CON, A), BTX (B), IGF1 (C), or GDNF (D) was daily applied onto the CAM for 3 d. E) On E7, mitochondrial morphology was examined, and MFI scores were quantified (E). F–J) After electroporation of DsRed-mito in E3 chick spinal cord, the hindlimb bud was removed (LBR; G) and either a same-stage limb bud (LBR+E3; H) or a 1-d-older limb bud (LBR+E4; I) was transplanted in place of the LBR. J) On E5.5, embryos were sampled, and MFI scores were quantified. n = 3 embryos/group. Scale bar = 10 μm.