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. 2013 Jan;27(1):187–198. doi: 10.1096/fj.12-208660

Figure 5.

Figure 5.

Gfap/Vim deletion does not alter expression or processing of APP- or Aβ-degrading proteases. A) RNA extracted from cortex of 12-mo-old mice was subjected to real-time PCR to compare transcript levels for APP processing secretases, lipoprotein mediators, and Aβ-degrading enzymes (as indicated), and expressed as fold-change compared to APP/PS1 Gfap+/+ Vim+/+ mice. Of all transcripts examined, only APH-1a and MMP9 showed differences between genotypes. *P < 0.05. B–D) Protein extracted from cortex of mice was analyzed by Western blot with anti-APP (B); anti-apoE and anti-apoJ (C); and anti-MMP-2 and anti-MMP-9 (D; pMMP-2, pro-MMP-2; pMMP-9, pro-MMP-9) antibodies as indicated. There was no significant difference in expression of any protein examined. Values are means ± se; n = 4–7 mice/group. E) Representative blots comparing APP/PS1 Gfap+/+ Vim+/+ (left lane) to APP/PS1 Gfap−/− Vim−/− (right lane) mice for each of the proteins above.