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. 2013 Jan;27(1):63–75. doi: 10.1096/fj.12-213801

Figure 1.

Figure 1.

ERα selectively regulates Orai3 expression. A) quantitative PCR (qPCR) data showing significant decrease in the transcript levels of ERα and Orai3 after transfection of MCF7 cells with siRNA targeting ERα (siERα; 72 h post-transfection) compared to siControl. Note that siERα does not affect Orai1 mRNA levels (n=4 independent transfections performed in duplicates). B) Western blot was performed with 100 μg of protein samples obtained from cells transfected with either siControl or siERα. Detection for ERα, Orai1, and Orai3 proteins was performed 96 h post-transfection using specific antibodies; β-actin loading control is also shown. C) Statistical quantification of Western blot data. *P < 0.05, **P < 0.01.