Figure 2.

ERα regulates Orai3-mediated SOCE in MCF7 cells. A) Western blot analysis showing that siERα was able to significantly reduce ERα expression. B) Statistical analysis of densitometry data from 4 independent transfections. C) Representative trace showing thapsigargin-activated SOCE in MCF7 cells measured using the standard Ca²⁺ off/Ca²⁺ on protocol simultaneously in several cells transfected with either siControl (n=17) or siERα (n=18) performed on the same day. D) Statistical analysis of Ca²⁺ imaging data from several independent runs (each representing several cells as shown in C). Note that in this figure and subsequent figures, the numbers in bar graphs (i.e., n=x, y) represent the total number of independent runs (x) and the total number of cells from all runs (y). E, F) Representative traces depicting the effect of siERα and siControl on 2-APB-mediated potentiation of SOCE (E) and statistical analysis on several independent experiments (F). G) Thapsigargin-activated SOCE was measured in MCF7 cells after 3 independent transfections with siControl, siERα, and siERα and eYFP-Orai3 cDNA plasmid. H) Statistical analyses on several independent runs similar to G. All data are derived from 3 or 4 independent transfections. *P < 0.05, **P < 0.01.