Figure 3.

ERα regulates Orai3-mediated CRAC currents in MCF7 cells. A, B) SiERα causes abrogation of CRAC currents measured in Ca²⁺-containing and DVF solutions, as well as 2-APB-mediated potentiation of these currents in comparison to siControl; data represented were taken from each ramp at −100 mV. C, D) I/V relationships of monovalent CRAC in MCF7 cells transfected with siControl or siERα before (C) and after (D) addition of 2-APB. I/V relationships are taken from traces in A and B where indicated by solid and shaded plus symbols and asterisks. E) Statistical analysis on several recordings (n=5–6) of monovalent CRAC currents and 2APB-mediated potentiation of these currents measured at −100 mV. **P < 0.01.