Figure 4.

ERα activation increases Orai3 expression and SOCE mediated by Orai3. A) Treatment with 100 nM 17β-estradiol increases Orai3 mRNA expression by 3-fold; data represent 4 independent experiments. B) Representative Ca²⁺ imaging traces showing that 17β-estradiol treatment increases SOCE, as well as 2-APB-mediated potentiation in MCF7 cells. C, D) Quantification of Orai3-mediated SOCE (C) and 2-APB-mediated SOCE potentiation (D) in MCF7 cells with or without 17β-estradiol treatment from 7 independent runs originating from 3 independent 17β-estradiol treatments. *P < 0.05, **P < 0.01.