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. 2013 Jan;27(1):63–75. doi: 10.1096/fj.12-213801

Figure 6.

Figure 6.

Orai3 knockdown inhibits SOCE-mediated NFAT activity and ERK1/2 and FAK phosphorylation in MCF7. A) NFAT activity assay was performed as described in Materials and Methods. Thapsigargin (2 μM for 15 min)-mediated NFAT activity was increased by siControl treatment, and this increase was significantly inhibited by siOrai3 treatment. Data are quantified from 3 independent experiments that were run in triplicates. B) PhosphoERK1/2 levels were measured in cell lysates containing proteases and phosphatases inhibitors before (time 0) and after thapsigargin stimulation for 5 and 15 min; ERK2 was used as a loading control. C) Ratio of densitometry of phosphoERK1/2 over ERK2; quantification of data obtained from 3 independent experiments. D) PhosphoFAK was also measured before (time 0) and after thapsigargin stimulation for 5 and 15 min, with total FAK used as loading control. E) Ratio of densitometry of phosphoFAK over total FAK; Quantification of data obtained from 3 independent experiments. *P < 0.05, **P < 0.01.