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. 2013 Jan;27(1):63–75. doi: 10.1096/fj.12-213801

Figure 7.

Figure 7.

Orai3 knockdown inhibits in vitro anchorage-independent growth and invasion in MCF7 cells. A) Western blot analysis showing decrease in Orai3 expression at 96 h after siOrai3 transfection. Densitometry data from 4 independent experiments are quantified in bar graph. B) Representative DAPI-stained fields showing decrease in MCF7 cell invasion on Orai3 knockdown in in vitro Matrigel invasion assays. Bar graph shows statistical analysis from 3 different transfections performed in triplicates, representing 12 fields/membrane. C) Western blot analysis showing decrease in Orai3 protein on infection with shOrai3-encoding lentiviruses compared to shNT-encoding lentiviruses. Densitometry data from 4 independent experiments are quantified in bar graph. D) Images from soft agar growth assay 4 wk after seeding of MCF7cells infected with shNT and shOrai3. Bar graph shows quantification of data obtained from 3 independent transfections performed in triplicate, representing 12 fields/condition. *P < 0.05, **P < 0.01.