Figure 8.

Orai3 knockdown inhibits breast tumor growth in vivo. A) Images of tumors in SCID mice injected with MCF7 cells infected with lentiviral particles encoding either shNT or shOrai3 at d 50 postinjection. B) Representative image of tumors isolated from shNT and shOrai3 groups at d 50 postinjection. C) Representative ultrasound scans on SCID mice injected with either shNT- or shOrai3- MCF7 cells in the mammary fat pad at d 45 postinjection; tumors are encircled in red. D) Tumor growth curve measured as increase in tumor volume as a function of time (d) postinjection of MCF7 cells (infected with either shNT- or shOrai3-encoding lentiviruses) into mammary fat pads of SCID mice. E) Scatterblot showing tumor weight at d 50 from all injected mice. F) Stable Orai3 knockdown was evaluated by Western blot on total tumor lysates from shNT and shOrai3 group at d 50 postinoculation. G) Densitometry data normalized to actin were quantified from 3 independent experiments. *P < 0.05, **P < 0.01.