Figure 4.

Intrahepatic CD11b⁺ Gr-1⁺ IMCs are increased in cryab^−/− mice during chronic liver inflammation. A) DEN-induced liver damage develops over a period of 30 wk after injection and results in foci of benign cellular alterations (dotted line). B) Semiquantitative scoring of foci of cellular alteration in the liver, as outlined in Materials and Methods (n=9 cryab^−/−; n=21 wild type). C) Microscopic image of an inflammatory focus in the liver 30 wk after DEN injection (arrow). D) Scoring of inflammatory foci in the liver as outlined in Materials and Methods (n=9 cryab^−/−; n=21 wild type). E) Immunofluorescence images of CD11b (red) and Gr-1 (green) staining in liver sections of wild-type and cryab^−/− mice. Scale bars = 100 μm. F) Quantification of CD11b⁺ Gr-1⁺ cells relative to liver area (n=9 cryab^−/−; n=21 wild type). Bars represent means ± sd. *P < 0.05.