Figure 3.

The aP2-lineage (RFP⁺) SVF cells express proliferation and adipose stem and progenitor cell markers. A–C) Immunostaining results of WAT and BAT SVF cells showing coexpression of RFP (red) and the proliferation marker Ki67 (green) in many cells. D–F) Coexpression of RFP (red) and the progenitor cell marker CD34 (green). G–I) Coexpression of RFP (red) and the stem cell marker Sca1 (green). J–L) Coexpression of RFP (red) and the preadipocyte marker Dlk1 (green). Nuclei are counterstained with DAPI (blue). Green Arrows indicate RFP and progenitor marker double positive cells. Error bars = se; n = 3. Scale bars = 100 μm.