Figure 6.

Ablation of aP2-lineage SVF cells inhibited adipogenic differentiation and gene expression. BAT SVF cells isolated from aP2-Cre/Rosa-iDTR mice were treated with vehicle control or DT that ablates aP2-lineage cells. Cells were grown to confluence and induced to differentiate. A) Oil red O signal of control (Con) and DT-treated cells after differentiation. B, C) mRNA and protein levels of aP2 in control and DT-treated SVF cells. D–F) Expression of adipogenic genes in control and DT-treated SVF cells after induced differentiation. Error bars = se; n = 3.