Figure 3.

Hepatic ATGL deficiency normalizes glucose intolerance without affecting hepatic insulin signaling in DIO mice. A, B) Serum glucose (A) and insulin (B) levels were measured 7 d after control or Atgl shRNA administration in mice fed control or HF diets for 12 wk (n=5). C) OGTT and corresponding AUC graph (n=5). D) At 7 d after adenovirus delivery of control or Atgl shRNA, mice underwent 4 h of food withdrawal and were injected intraperitoneally with saline or insulin at 1 U/kg body weight and were euthanized 10 min later. Hepatic insulin signaling was assessed by immunoblotting to determine insulin-stimulated phosphorylation of Akt, GSK-3α, and IRS1 (n=3–5). *P < 0.05 vs. control shRNA group; ^#P < 0.05 vs. control diet group or saline-injected group.