Table 2.
Characteristics of 18 nelwly developed SSR markers in pineapple
| SSR locus Accession nos.a |
Primer sequence (5′-3′) | Repeat motif | Target size (bp) | No. of allels | (HE) Heterozygosity |
|---|---|---|---|---|---|
| TsuAC004 AB716708 |
F: ATGTTGGTCAAAGGGCTGTT R: gtttcttTCATGATCACACTGGAGATTTG |
(AG)16 | 144 | 5 | 0.67 |
| TsuAC007 AB716709 |
F: GCAGCGGTAAGATCTGCTTT R: gtttcttTCCTTCTCTCCACCTCTTCATT |
(GA)21 | 102 | 4 | –b |
| TsuAC008 AB716710 |
F: GAAATGGTACTGCTTCACTGTTC R: gtttcttATACGGGGAAATAGGCACAA |
(GA)16 | 173 | 5 | 0.71 |
| TsuAC010 AB716711 |
F: TGAGTTGTGTCATTGTGTGTCA R: gtttcttGGGGGTCTCCATACATTTTT |
(GT)14A(AG)12 | 207 | 7 | 0.76 |
| TsuAC013 AB716712 |
F: TTATGCAGGAAAATAGGGGG R: gtttcttCATGCATCATAAATTCGTGTCC |
(AGAGAT)3(AG)12 | 139 | 4 | 0.55 |
| TsuAC018 AB716713 |
F: GCATCGATCTCCATGCAAAC R: gtttcttAAAGGAAACAAGGAGGATGTGA |
(CA)10A(AC)9 | 120 | 5 | 0.59 |
| TsuAC019 AB716714 |
F: TTCATCCTATGGTTTCCCCA R: gtttcttGTGGGTTCAACTGAGTAGCAAT |
(AC)13 | 177 | 4 | 0.09 |
| TsuAC021 AB716715 |
F: AATCAAAGTGATTCCCCTTCC R: gtttcttTCTGACATAGGGCTTGCACA |
(CA)21 | 141 | 4 | 0.50 |
| TsuAC023 AB716716 |
F: TCGAAAAGAGGATGCTGGAT R: gtttcttTCCGCAGTGTAGGCATGTAA |
(CA)10(TA)11 | 143 | 5 | 0.73 |
| TsuAC024 AB716717 |
F: GTCGCCAATCAAATTCCAGT R: gtttcttCTCACGAAACATGAATCACCA |
(AC)9 | 126 | 3 | 0.52 |
| TsuAC026 AB716718 |
F: GGGATTAACTTTTCCAGGGG R: gtttcttTTGGATTCCTCGTTTGCATT |
(AC)8 | 200 | 4 | 0.09 |
| TsuAC028 AB716719 |
F: TGACACCATAGAGGAGGGGT R: gtttcttGCTCAAGGACAATCCACCAT |
(AC)8 | 220 | 3 | 0.57 |
| TsuAC030 AB716720 |
F: GAGAGAGAAAAGAGTTTCGACAG R: gtttcttCTTCAAAATGGTCTAACGTACC |
(AG)27 | 149 | 4 | 0.43 |
| TsuAC035 AB716721 |
F: TTCCTAGCCAACACTACTACAGA R: gtttcttTGCAGCTTCTTTTCCTGGTT |
(GA)9 | 96 | 3 | 0.45 |
| TsuAC038 AB716722 |
F: TTGCAGCAAACCAAGTCAT R: gtttcttGGAGGTGTAGTCAATTAGGAGAA |
(AC)11 | 327 | 3 | 0.48 |
| TsuAC039 AB716723 |
F: CCCTGTATGGGTAGCATTGAA R: gtttcttAAAAGGTATCACGAAAGCGA |
(AC)8 | 91 | 3 | 0.54 |
| TsuAC040 AB716724 |
F: AAATTCTCTTCATGCACACG R: gtttcttTGCTTCATGAGATCTAAACTGG |
(AC)8 | 99 | 4 | 0.61 |
| TsuAC041 AB716725 |
F: CTCTCTTATGGCACAACCCTG R: gtttcttCCTGGTGAGTAATCTATATGCTG |
(AC)11 | 279 | 4 | 0.58 |
| Average | 4.1 | 0.52 |
a
DDBJ accession numbers
b
Heterozygosity not evaluated because of the existence of null allele