Figure 6. Confirmation of the miR-451 phenotype using miR-451^null dendritic cells and gain of function studies.

(A) Primary splenic dendritic cells isolated from wild type or miR-451^null mice were infected with influenza for 24 hours and secreted cytokine levels were quantified by ELISA. Means ± SEM for n≥3 are shown. (B) Splenic dendritic cells were treated as described in A, RNA isolated after 6 h or 18 h, qRT-PCR performed, and the expression of Il-6, Tnf, and Ifn-β was plotted relative to EF-1 expression. Means ± SEM for n=3 are shown. (C) JAWS II dendritic cells over-expressing miR-451 or vector alone were infected with the influenza A strains PR8 or X31 for 24 h and secreted cytokines were quantified by ELISA. Means ± SEM for n=3 are shown. (D) Bone marrow-derived dendritic cells over-expressing miR-451 or vector alone were infected with the influenza A strain PR8 or stimulated with poly(I:C) for 21 h and secreted cytokines quantified by ELISA. Means ± SEM for n=3 are shown; **p<0.01.