Fig. 2.

TCRαβ + CD8αβ + T cells are the dominant population of lymphocytes in the conjunctival epithelium. Representative scatter profile of leukocyte populations derived from matched peripheral blood and conjunctival OSIC from a healthy subject. Data are shown for a representative subject, gated on CD45+ live cells for peripheral blood (a) and conjunctival OSIC (b). CD45RO staining is shown for CD3 + CD56 − TCRγδ − gated CD4 + CD8β − (top panel) and CD8αβ + (bottom panel) cells. Percentages of CD3 + CD56- lymphocytes are shown for the representative subject (n = 30). Statistical comparisons between peripheral blood and conjunctival impression populations of CD8αβ+, CD4+, CD8αα+ and TCRαβ+CD4 − CD8αβ− (double negative; DN) T cells (c–f) were undertaken by the Mann–Whitney U test (NS not significant [P > 0.05], **P < 0.01, ***P < 0.001)