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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: J Pathol. 2011 Aug 24;225(3):378–389. doi: 10.1002/path.2962

Figure 5.

Figure 5

Enhancement of vIL6 or hIL6 expression by specific miRNA inhibitors. (A and B) Inhibition of endogenous miR-1293 or miR-608 in HEK293 cells to promote expression of vIL6 or hIL6. HEK293 cells were co-transfected with 200 nM of indicated anti-miRs along with 100 ng of FLAG-vIL6 (Left) or FLAG-hIL6 (Right). Protein samples and total RNA were prepared 24 h after transfection. Protein samples were analyzed by Western blot with anti-FLAG antibody for vIL6 or hIL6 expression or anti-β-tubulin antibody for sample loading (A). The amount of vIL6 or hIL6 RNA in the corresponding RNA samples was analyzed by qRT-PCR assays (B). Bars represent means ± SD (n=3). ** and * indicate P<0.01 and P<0.05 (t-test), respectively (B). (C and D) Inhibition of endogenous miR-214 did not affect vIL6 or hIL6 expression (C), but ectopic expression of miR-214 did do (D). See other details in (A).

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