Figure 7.

Distribution of endogenous miR-1293 and vIL6 in lymph nodes. In situ hybridization was performed on lymph node sections from a patient with HIV-associated folicular hyperplasia (non-MCD, A–I) or with MCD (J–R) by using no specific probe (A–C and J–L) or a miR-1293 detection probe (D–F and M–O). The purple color indicates miR-1293. After NBT/BCIP reaction, tissue sections were counterstained with the FastRed nuclear staining reagent. The same tissue sections on separate slides were immunostained by an anti-vIL6 mouse monoclonal antibody for vIL6 (blue color) protein expression (G–I and P–R). MZ, mantle zone; GC, germinal center. (B, E, H, K, N and Q), a representative region showing both MZ and GC from (A, D, G, J, M and P), respectively, was imaged at higher magnification. (C, F, I, L, O and R), the regions in the box of (B, E, H, K, N and Q) were further enlarged, respectively. The images in (N )and (O) were adjusted for better contrast of the fastRed in MZ. Scale bars represent 200, 50 and 20 μm from the left to the right in each panel sets of three.