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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: Mamm Genome. 2011 May 25;22(9-10):506–517. doi: 10.1007/s00335-011-9333-7

Figure 3.

Figure 3

Levels of chromatin-bound PCNA, Pol δ and the MCM2-7 proteins are significantly reduced in B6 Mcm4chaos3/chaos3 (C3/C3) MEFs. Reduction rates for chromatin-bound MCM2/4/7 and the other replication proteins in Mcm4chaos3/chaos3 cells were estimated by referencing wildtype (WT) proteins loaded in different amounts (left). In Mcm4chaos3/chaos3 cells, the MCM3/5/6 proteins were also reduced in both the chromatin fraction and the total cell lysate (right). Note that upregulation of p21 also occurs in Mcm4chaos3/chaos3 cells. Actin was used as a loading control. Samples were harvested at Day 2 (Fig. 1D) when there was no difference in proliferation between B6 wildtype and Mcm4chaos3/chaos3 cells.