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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: Mamm Genome. 2011 May 25;22(9-10):506–517. doi: 10.1007/s00335-011-9333-7

Figure 4.

Figure 4

C3H and F1 Mcm4chaos3/chaos3 cells proliferate normally despite reduced levels of the MCM 2-7 proteins, but activate p53. A) MCM7 protein levels were significantly reduced both in total cell lysates and chromatin fractions in B6, C3H and F1 Mcm4chaos3/chaos3 (C3/C3) cells compared to their respective wildtype (WT) cells. However, the reduction of chromatin-bound PCNA was most severe in B6 Mcm4chaos3/chaos3 cells. B) F1 and C3H Mcm4chaos3/chaos3 MEFs proliferate at the same rates as their respective wildtype control cells in vitro. Bars show standard deviations. C) Regardless of p21 expression levels, strong p53 activation is observed in F1 and C3H Mcm4chaos3/chaos3 cells. Note that the level of p21 expression appears to be intrinsically lower in B6 wildtype cells.