Figure 3.

Improvement of L-cystine production by NrdH or Grx1 in vivo. L-cysteine plus L-cystine production by L-cysteine producer with plasmid overproducing Grx1 or NrdH genes. BW25113 harboring the L-cysteine over producing plasmid pDES (L-cysteine overproducer) was transformed empty vector pCA24N (circles), pGrx1 (triangles) or pNrdH (squares). These strains were cultivated in SM1 medium with 30 mM thiosulfate for 48 hours. The concentration of L-cysteine plus L-cystine was determined by Gaitonde method. Values indicate means and standard deviations of results from three independent experiments.