Figure 6.

Effect of L-cysteine production by enhancement of the sulfite reductases and/or the L-cysteine synthases. L-cysteine overproducer was transformed (a) empty vector pCA24N (closed circles), pCysI (opened triangles), pCysJ(opened squares), pCysG (opened diamonds) and pCysK (opened circles), (b) pCA24N (closed circles), pCysI-K (opened triangles), pCysJ-K (opened squares) and pCysI-J (opened diamonds) to promote the incorporation of sulfite reduced from SSC to the sulfate pathway. These strains were cultivated in SM1 medium with 30 mM thiosulfate for 36 hours. The concentration of L-cysteine plus L-cystine was determined by Gaitonde method. Values indicate means and standard deviations of results from three independent experiments.