Table 1.

Bacterial strains and plasmids used

E. coli strain or plasmid	Genotype	Reference or source
Strains
BW25113	wild type	[21]
JW2720	cysC::KmR	[21]
JW3582	cysE::KmR	[21]
JW3331	cysG::KmR	[21]
JW2732	cysH::KmR	[21]
JW2733	cysI::KmR	[21]
JW2734	cysJ::KmR	[21]
JW2407	cysK::KmR	[21]
JW2414	cysM::KmR	[21]
AG1	$recA1endA1gyrA96thi-1hsdR17\left(r_K{-m}_K{}^{+}\right)supE44relA1$	[22]
Plasmids
pDES	pACYC184 with serA (T410 Stop), ydeD and alterd cysE (T167A) genes under the control of the OmpA promoter	[9]
pCA24N	CmR	[22]
pCysH	pCA24N, cysH gene on 0.7 kb DNA fragment	[22]
pCysI	pCA24N, cysI gene on 1.7 kb DNA fragment	[22]
pCysJ	pCA24N, cysJ gene on 1.8 kb DNA fragment	[22]
pCysK	pCA24N, cysK gene on 0.9 kb DNA fragment	[22]
pCysM	pCA24N, cysM gene on 0.9 kb DNA fragment	[22]
pGrx1	pCA24N, grxA gene on 0.26 kb DNA fragment	[22]
pGrx2	pCA24N, grxB gene on 0.62 kb DNA fragment	[22]
pGrx3	pCA24N, grxC gene on 0.25 kb DNA fragment	[22]
pGrx4	pCA24N, grxD gene on 0.35 kb DNA fragment	[22]
pTrx1	pCA24N, trxA gene on 0.33 kb DNA fragment	[22]
pTrx2	pCA24N, trxC gene on 0.42 kb DNA fragment	[22]
pNrdH	pCA24N, nrdH gene on 0.25 kb DNA fragment	[22]
pCysK-I	pCA24N, cysK, cysI gene on 2.6 kb DNA fragment	This study
pCysK-J	pCA24N, cysK, cysIJgene on 2.7 kb DNA fragment	This study
pCysJ-I	pCA24N, cysJ, cysI gene on 3.5 kb DNA fragment	This study
pCysK-I-NrdH	pCA24N, cysK, cysI, nrdH gene on 3.0 kb DNA fragment	This study