Table 3.
Primers used for validation of stress-responsive genes by RT-qPCR
| Gene ID | Symbol | Sense (5′-3′) | Antisense (5′-3′) | Amplicon size (bp) |
|---|---|---|---|---|
| 18S ribosomal RNA |
Rn18s |
CCCGAAGCGTTTACTTTGAA |
CCCTCTTAATCATGGCCTCA |
136 |
| Glyceraldehyde-3-phosphate dehydrogenase |
Gapdh |
GAAGGGCTCATGACCACAGT |
GGATGCAGGGATGATGTTCT |
117 |
| Actin, beta |
Actb |
CACACTGTGCCCATCTATGA |
CCGATCGTGATGACCTGACC |
272 |
| Calcium/calmodulin-dependent protein kinase II alpha |
Camk2a |
GCCTGGACTTTCATCGATTC |
GGTACTGAGTGATGCGGATGT |
141 |
| Glycogen synthase kinase 3 beta |
Gsk3β |
GCGAGACACACCTGCCCTCTTC |
GTGGCCAGAGGTGGGTTACTTGAC |
66 |
| Mammalian target of rapamycin (serine/threonine kinase) |
mTOR |
TTGGATGTTCCAACCCAAGT |
CAGGCCTTGGTTACCAGAAA |
106 |
| Neurotrophic tyrosine kinase, receptor, type 2 |
Ntrk2 |
TGGAGGGCGACCCACTCATCA |
TCAGCTCGG TGGGCGGGTTA |
123 |
| Neurotrophic tyrosine kinase, receptor, type 3 |
Ntrk3 |
CATCCGCTGGATGCCACCTGAAA |
AAGACACGGCCTTGGGTGATGCA |
50 |
| Phosphoinositide-3-kinase, catalytic, beta polypeptide |
Pik3cb |
CCTGCGACAGATGAGTGATG |
CAATCCTCCGGTTGTCAAGT |
134 |
| V-raf murine sarcoma viral oncogene homolog B1 |
Braf |
CATGGCGACGTGGCAGTGAAAATG |
TGAGGTGTGGGTGCTGTCACATTC |
50 |
| Glyceraldehyde-3-phosphate dehydrogenase-3-prime |
Gapdh-3′ |
GGCTGGCATTGCTCTCAA |
GAGGTCCACCACCCTGTTG |
88 |
| Glyceraldehyde-3-phosphate dehydrogenase-5-prime |
Gapdh-5′ |
GACAGCCGCATCTTCTTG |
CACCGACCTTCACCATCTTG |
63 |
| Actin, beta-3-prime |
Actb-3′ |
CCTAGCACCATGAAGATCAAGA |
GCCAGGATAGAGCCACCAATC |
77 |
| Actin, beta-5-prime | Actb-5′ | ACCCAGATCATGTTTGAGACCTT | CAGAGGCATACAGGGACAAC | 79 |