Table 1.
MICs of the extract, fractions, compounds from Dioscorea bulbifera and chloramphenicol on documented strains and clinical MDR isolates
|
Samplesa |
Microorganisms and MIC(μg/mL)without and in the presence of PAßN(in parenthesis)b |
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
E.coli |
E.aerogenes |
K.pneumoniae |
P.aeruginosa |
M.smegmatis |
M.tuberculosis |
|||||||||
| ATCC8739 | AG100A | AG102 | ATCC13048 | EA289 | EA-CM64 | ATCC11296 | KP55 | KP63 | PA01 | PA124 | ATCC 700084 | ATCC 27294 | MTCS1 | MTCS2 | |
| DBB |
64 (64) |
64 (32) |
128 (128) |
64 (64) |
256 (128) |
256 (256) |
64 (64) |
128 (128) |
128 (128) |
128 (128) |
128 (128) |
64 |
64 |
256 |
64 |
| DBB1 |
32 (32) |
16 (8) |
128 (128) |
32 (32) |
64 (64) |
64 (64) |
32 (32) |
64 (64) |
64 (64) |
64 (64) |
64 (64) |
3x2 |
64 |
256 |
32 |
| DBB2 |
64 (64) |
16 (8) |
64 (64) |
32 (16) |
64 (64) |
64 (64) |
64 (64) |
64 (64) |
32 (16) |
32 (16) |
64 (64) |
32 |
32 |
64 |
16 |
|
1 |
256 (64) |
128 (32) |
- |
256 (128) |
- (256) |
- |
256 (64) |
512 (128) |
- (256) |
- (512) |
- |
- |
nd |
nd |
nd |
|
2 |
64 (32) |
64 (64) |
64 (32) |
128 (64) |
64 (64) |
64 (64) |
64 (32) |
128 (64) |
64 (32) |
128 (64) |
128 (64) |
32 |
32 |
32 |
16 |
|
3 |
64 (16) |
16 (8) |
64 (16) |
64 (16) |
64 (16) |
64 (16) |
64 (32) |
64 (16) |
64 (32) |
128 (32) |
128 (32) |
8 |
8 |
32 |
8 |
|
4 |
64 (64) |
64 (32) |
64 (64) |
128 (32) |
64 (32) |
128 (64) |
64 (64) |
64 (64) |
64 (32) |
128 (64) |
128 (64) |
32 |
64 |
64 |
32 |
|
5 |
64 (64) |
64 (32) |
128 (64) |
128 (64) |
128 (32) |
256 (64) |
128 (128) |
128 (64) |
256 (64) |
256 (64) |
128 (32) |
64 |
32 |
64 |
32 |
|
6 |
256 (256) |
256 (256) |
- (512) |
- (512) |
- (256) |
- |
256 (128) |
- (256) |
- (256) |
- (256) |
- (512) |
- |
nd |
nd |
nd |
| RA | 4 (1) | 1 (0.5) | 16 (1) | 4 (1) | 512 (128) | 256 (8) | 2 (2) | 32 (4) | 512 (128) | 128 (8) | 256 (8) | 0.5 | 0.5 | 64 | 2 |
aTested samples [DBB: methanol extract from the bulbils of D. bulbifera; bafoudiosbulbins A (1), B (2), C (3), F (4), G (5), 2,7-dihydroxy-4-methoxyphenanthrene (6); EtOAc fraction (DBB1) and EtOAc sub-fraction C (DBB2); RA : reference antibiotics were chloramphenicol for bacteria, ciprofloxacin for M. smegmatis, isoniazid for M.tuberculosis; (−): MIC > 512 μg/mL; bThe drugs and compounds were tested in the absence or in the presence (values in braket) of PAßN at a final concentration of 20 μg/mL, as described previously [7]. At this concentration, no intrinsic effect against the various bacterial strains (included as internal controls in each assay without antibiotic) was observed.