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Expression and functional role of NKA mutant E961A. (A) E961A mutant appears localized at the membrane. (B) Functional experiments with the E961A mutant: Phospholemman reduces the pump’s apparent affinity for intracellular Na+ (increases K0.5), but PLM phosphorylation does not affect the function of the E961A–NKA mutant (error bars indicate SEM; n = 4–5).
