Fig. 3.

Single-cell–based hierarchical reconstruction of innate immune response signaling states. (A) Hierarchical clustering of single cells grouped at left based on virus-derived replication states and at right (horizontal dotted lines) on category 1 transcripts, groups as shown in B. Assay columns from left to right are in the order shown in Table S2. (B) Antiviral states in different subpopulations following RV infection of IECs. NF-κB–dependent transcripts from category 7 are indicated by asterisks. Statistically significant changes (P < 0.05) relative to population-matched uninfected cells in the positive (blue) or negative (red) direction are indicated. Numbers refer to the transcript categories in Fig. 2B.