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. 2012 Nov 21;109(50):20401–20406. doi: 10.1073/pnas.1211532109

Fig. 1.

Fig. 1.

DNA polymerase δ incorporates both A and C opposite 8-oxo-G, but displays difficulties when extending from C:8-oxo-G base pairs. (A) Schematic depiction of the primer-extension reactions. The sequence context of the template allows incorporation of C or A only opposite 8-oxo-G, as the rest of the sequence consists exclusively of C and A. (B) Selectivity of pol δ to incorporate A or C opposite 8-oxo-G and the influence of PCNA on this reaction. PCNA in indicated amounts was titrated to 1 nM pol δ on 8-oxo-G (SS) template (lanes 1–10) or on 8-oxo-G (RS) template (lanes 11–20) in the presence of either dATP + dGTP + dTTP (lanes 15–17) or dCTP + dGTP + dTTP (lanes 18–20).