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. 2012 Nov 21;109(50):20401–20406. doi: 10.1073/pnas.1211532109

Fig. 4.

Fig. 4.

Switch between the replicative DNA polymerase δ and the TLS DNA polymerase λ influences the correct bypass of 8-oxo-G lesions both in cellular extracts and in vivo. (A) Activity of MEF pol λ+/+ or pol λ−/− extracts on RS primer extension assays in the absence or the presence of recombinant pol δ. All reactions contain dGTP + dTTP. (B) Quantification of full-length product in A in the presence of recombinant pol δ. The full-length signal was normalized to the total intensity of the polymerization products and the amount of full-length product formed by MEF pol λ+/+ extracts in presence of A. Values are the results from three independent experiments showing mean ± SD. The P value was calculated using the Student’s t test. (C) Sequence analysis of gapped plasmids extracted from MEF pol λ+/+ or pol λ−/− cells. The absolute numbers of the respective mutations followed by the total amount of analyzed sequences are indicated in parentheses. (D) Sequence analysis of gapped plasmids extracted from HeLa cells treated with siRNA against pol λ or an unspecific siRNA. The absolute numbers of the respective mutations followed by the total amount of analyzed sequences are indicated in parentheses.