Skip to main content
. 2012 Nov 19;109(50):E3444–E3453. doi: 10.1073/pnas.1214024109

Fig. 4.

Fig. 4.

The lysC riboswitch can be directed to control either translation initiation or mRNA decay. (A) β-Galactosidase assays using translational (trL) and transcriptional (trX) fusions of the lysC riboswitch for the wild-type, ∆Site1, ∆Site2, and ∆Site1-2 constructs. β-Galactosidase enzymatic activities were measured in the absence and presence of 10 µg/mL lysine. Values obtained for translational and transcriptional fusions were normalized to enzymatic activities obtained for WT translational and transcriptional fusions, respectively, in the absence of lysine. See SI Appendix, Fig. S2 for details about riboswitch mutant constructs. (B) β-Galactosidase assays using translational and transcriptional fusions of the lysC riboswitch for the wild-type, AAG, and GACG constructs. Values obtained for translational and transcriptional fusions were normalized to enzymatic activities obtained for WT translational and transcriptional fusions, respectively, in the absence of lysine. See SI Appendix, Fig. S2 for details about riboswitch mutant constructs.