Fig. 5.

Interchange between CP conformations involves subunit interfaces. (A) Each β-subunit (gray) is adjacent to a pair of α-subunits (lime) and pairs of β-intra-ring (dark blue) and β-inter-ring (purple) subunits. (B) β-Subunit methyl groups with the largest chemical shift differences (norm) between WT and αL81V CPs are within 12 Å of the α-ring. (C) β-Subunit chemical shift differences (WT vs. αL81V CPs) are largest for methyls at α/β interfaces, with some differences also at intra-β/β interfaces (methyl groups >12 Å from an interface are defined as “not near an interface”). (D–F) As above, but for the α-subunit, with norms computed as chemical shift differences of corresponding α-subunit methyl groups on WT and βT1A-pro CPs.