Fig. 6.

Relative populations of CP conformations modulate the distribution of cleavage products. (A) Reverse phase HPLC trace of cleavage products generated with different CP variants (0.3 μM WT, αL81A, αL81V, and 11S-bound) incubated with 300 μM α-synuclein for 30 min at 50 °C. (B) Comparison of reverse phrase HPLC profiles of α-synuclein degradation products shows that similar products are produced with the CP variants but that the relative amounts of each differ. Expansions of traces are shown for peaks 2, 3, and 5. mAu, milli Absorbance unit (UV-visible spectroscopy).