Fig. 3.

Device validation. Neutrophils (blue) and monocytes (red) dynamically migrate to different chemoattractants. Graphs correspond to average cell counts in all FCCs (n = 16). (A–F) MCP-1 (100 nM) (A), IL-8 (10 nM) (B), fMLP (100 nM) (C), negative control (D), LTB₄ (100 nM) (Movie S2) (E), and LXA₄ (100 nM) (F). (G) Dose–response of LXA₄ as a chemoattractant to monocytes. Experiments were repeated with blood from three healthy donors for each condition. (H) Monocyte counts vs. time for two different-sized (6.6 vs. 2.6 nL) FCCs. The t_1/2 was ∼5 h for both chambers (5 vs. 5.25), and the t_max occurred at 13 h (45 cells) and 9 h (28 cells) for the large and small FCCs, respectively.