Fig. 5.

Expression levels of the two duplicated genes Evx2 and Lnp. (A) Schemes of the various genetic configurations. (B and C) RT-qPCR analysis of Lnp and Evx2 expression levels in E12.5 developing digits dissected from embryos homozygous for the various rearrangements. For each allele, the number of copies of these two genes is indicated above the graphs. Both genes are clearly expressed at higher levels in the Dup(Nsi-Atf2) allele but not other configurations, where they are present in two copies, indicating an influence of genomic topology rather than a mere consequence of copy number. Lnp and Evx2 mRNAs levels do not correlate with the impact of these modified configurations on Hoxd gene expression. The WT levels are set to one for each gene. Error bars indicate SD (n = 4).