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. 2012 Nov 20;109(50):20497–20502. doi: 10.1073/pnas.1217018109

Fig. 1.

Fig. 1.

Repaglinide inhibits sperm chemotaxis. (A) Sperm trajectories toward a SAAF-filled capillary (red) in the absence (Upper) and presence (Lower) of 150 µM repaglinide. (Scale bar, 100 µm.) Trajectories of three representative sperm are shown (Right). (B) Quantitation of chemotaxis using LECI. n = 12–28. (C) Repaglinide does not significantly alter sperm swimming velocity during chemotaxis. n = 30. (D) Pseudocolor display of [Ca2+]i as sperm swim toward a chemoattractant in the absence (DMSO) or presence of 150 µM repaglinide. Sperm trajectories and the position relative to chemoattractant (red) are shown in pseudocolor display of flagellar part (Right). (E) Maximum Fluo-8H fluorescent intensity of flagellar part during chemotaxis. Repaglinide treatment does not alter the maximum [Ca2+]i. n = 12–21. Arrows in A and D indicate direction of movement. *P < 0.01 vs. 0 µM; **P < 0.001 vs. 0 µM.